Instrument Listing

The GE Healthcare ImageQuant LAS 4000 (MIC room 310) is a digital imaging system for sensitive, quantitative imaging of gels, blots, and colonies.

• Exceptional sensitivity for quantitation of biomolecules labeled with chemiluminescent probes, and expandable to multifluorescent UV, visible, and IR imaging. Allows gel documentation of Coomassie and silver-stained samples
• Accurate quantitation of closely spaced bands in complex protein samples for gels and films up to 21 x 14 cm (25 x 25 cm using the optional lens). Low noise for longer exposure times and lower background, important for precise quantitation of very weak signals
• Wide linear dynamic range for precise quantitation of faint and dark bands over four orders of magnitude in the same image, avoiding time consuming multiple film exposures
• Distortion, dark frame, and flat frame corrections are applied to each imaging mode for optimal precision and uniform quantitation
• Sensitive detection of less than 40 pg of protein on a Western blot. Sensitivity may be increased by “binning” of up to 8 x 8 pixels and smoothing
• Chemiluminescent and colorimetric signals can be captured in the same image without changing the lens. Optional multifluorescence detection extends functionality to the capture of fluorophores such as Cy2, Cy3, and Cy5 and Deep Purple Total protein stain

ImageQuant LAS 4000 is designed to quantitate the highly sensitive detection of proteins using Amersham ECL Western blotting reagents. A free combination of light sources and filters can be incorporated into the imager according to your needs.

The system consists of an intelligent dark box, camera, lens, and automated four-position filter wheel with a standard filter for EtBr and open positions for the filters delivered with the optional fluorescent light sources. Focusing, filters, illuminators, and exposure time are remotely controlled by a computer. Simple operation allows adjustment without having to open the cabinet. The intelligent light box provides automation and user safety. UV light sources are housed inside the cabinet, and can only be switched on when the door is closed.

A bright, wide aperture FUJINON F0.85 lens, specially developed for chemiluminescence imaging, projects sharp images onto a patterned CCD chip. By diagonally aligning octogonal pixels, the gap between pixels in the horizontal and vertical planes is reduced. This pattern allows an effective image resolution of 6.3 Mpixels.

The user-friendly control software has several capture modes for achieving optimal sensitivity and dynamic range including increment, repetition, and program modes. For image analysis, advanced and flexible ImageQuant TL software is available separately and comprises a dedicated suite of analysis applications to provide high levels of automation and accuracy in analysis of gels, blots, and colony counting.

Thermo  Eclipse Tribrid

The Thermo Eclipse  (located in MIC rm 109)** is equipped with a Thermo Vanquish Neo LC system, allowing nano and cap LCMS . Features tribrid architecture including a quadrupole mass filter, dual pressure linear ion trap and an Orbitrap Mass analyzer. High mass range MSn (HMRⁿ) mode for intact protein analysis. FAIMS Pro interface.

• Resolving power of >500,000 FWHM at m/z 200

• Multiple fragmentation techniques:  CID, HCD, ETD

**PLEASE CONTACT TERENCE WU FOR INFORMATION

The Agilent 6550 Q-TOF LC/MS System,(MIC room 109) uses iFunnel technology to deliver the lowest detection levels of any high resolution LC/MS instrument currently available.  Low femtogram-level sensitivity with high resolution across a relatively broad mass range (as compared to Orbitrap) as well as accurate mass make it a versatile platform, and a good choice for small molecule/metabolite ID, metabolomics and proteomics applications. We currently have from Agilent a 1290 LC system coupled to the dual spray API source.

iFunnel increases ion transfer to achieve the lowest detection levels of any high resolution LC/MS instrument, achieving low femtogram-level sensitivity

Ion Beam Compression and Shaping (IBCS) technology provides the greatest sensitivity while maintaining 40k mass resolution, sub ppm mass accuracy, and a faster scan rate up to 50 spectra per second.

Agilent Jet Stream technology combined with improved ion optics provide 5x greater on-column sensitivity and increase up time to maintain the highest sensitivity for high throughput applications.

In-spectrum dynamic range of up to 5 orders of magnitude reveals trace-level targets, even in the presence of much more abundant compounds

The Agilent Cary 660 FTIR (MIC room 119) is a versatile, high performance spectrometer that meets a broad range of routine spectrometry and research analysis. The instrument is controlled using high performance operating software and can also be fitted with a wide range of accessories to provide extra capabilities. The core labs 660 is equipped with a Pike Technologies GladiATR™ Single Reflection ATR accessory. The common platform of Agilent’s Cary 660/670/680-FTIR spectrometers enables the performance and capabilities of the imaging system utilized to be scaled.

The FTIR instrument is located in MIC 119. Login is with Yale Net ID and password.  It is equipped with an ATR with Diamond crystal.

Operational characteristics include resolution of 0.0001 degree, accuracy of +/- 0.002degree and up to 8 measurable wavelengths. Peltier temperature control is also provided.

BD FACSAria:

We currently have three laser lines (640 nm, 561 nm 488 nm, 408 nm capable of 15 color sorting).

The Red laser (640-nm):

1.      780/60; 750LP APC-CY7

2.      670/30; APC

The Yellow-green laser (561-nm):

1.     670/30; 635LP: PE-Cy5

2.     780/60; 750LP: PE-CY7

3.     610/20; 595LP: m-cherry

4.     585/15; 550LP: PE

5.     485/22 FCS PMT

The Blue laser: (488-nm):

1.      710/50; 685LP: PerCP-CY5.5

2.      530/30, 505LP: FITC OR GFP (495LP; 515/20)

3.      488/10: SSC

The Violet laser: (405-nm):

1.    710/50; 685LP: QDot705

2.    610/20; 595LP: QDot 605

3.    450/50: Pacific Blue

4.    780/60; 750LP: BV 786

5.    670/30; 635LP: Qdot 655

6.    530/30; 475LP Qdot525

The GE Healthcare Typhoon FLA 9500 (MIC room 310) is a variable mode laser scanner with modular access to the optical components, providing both versatile and flexible imaging for precise quantitation of proteins, nucleic acids, and other biomolecules.

• Versatility: imaging of multifluorescent-, chemifluorescent-, radioisotope-labeled, and colorimetric samples
• High resolution and quantitation: a pixel resolution of up to 10 µm and a linear signal response over five orders of magnitude provides precise quantitation in gels, blots, tissue sections, and arrays
• High sample throughput: scanning area of 40 × 46 cm enables simultaneous imaging of up to 20 gels or blots, measuring 10 × 8 cm in size. This facilitates comparisons among blots, and reduces workload and waiting time
• Flexibility: optimized performance for new applications by adapting the system with stages, detectors, filters and lasers
• 2-D DIGE imaging: simultaneously image two 2-D DIGE gels for differential expression studies
• Visible and infrared fluorescence imaging: optional near infrared excitation for imaging IRDyeTM and other infrared dyes

The system provides several imaging modes at any time, including fluorescence and chemifluorescence, filmless autoradiography, and digitization of colorimetrically-stained samples (e.g., CoomassieTM blue and silver stain). It is also adequate for quantitative chemiluminescent imaging that does not require maximum sensitivity.

The system is optimized for 2-D DIGE imaging for differential protein expression studies, ECL Plus chemifluorescent imaging for quantitative protein detection by Western blotting, and multifluorescent ECL Plex imaging for precise quantitation of two or more proteins in the same blot.

Typhoon FLA 9500 comes equipped with both bi-alkali and multialkali photomultiplier tubes (PMT). This combination provides excellent detection over a very broad spectrum. Each PMT is selected for optimal response to the detected emission wavelength.

The system accommodates four automated filters. Filters are easily accessed and exchanged without tools for attaining optimal imaging conditions.

Stages give the optimal imaging conditions for a range of samples. Samples include agarose and polyacrylamide gels, membranes, DIGE gels, radioisotope-labeled samples, as well as microplates and glass slides with the TP plug-in. The system can simultaneously scan two DIGE gels, each measuring up to 20 x 25 cm. The stages are easily removed from the system for cleaning.

A fast 1000 μm prescan function gives a rapid overview of the sample. The system provides a linear signal response over five orders of magnitude, providing a suitable basis for precise quantitation.

Lasers can be exchanged in the field to accommodate new applications and fluorophores.